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M94A3189.TXT
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1994-10-25
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Document 3189
DOCN M94A3189
TI The growth potency and cytopathogenicity of recombinant chimeric viruses
between HIV-1 and SIVagm.
DT 9412
AU Jin MH; Ido E; Kuwata T; Igarashi T; Okada M; Cichutek K; Kurth R; Miura
T; Hayami M; Inst. for Virus Res., Kyoto Univ., Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):132 (abstract no. PA0146). Unique
Identifier : AIDSLINE ICA10/94369386
AB OBJECTIVE: We attempted to identify the viral determinant for
pathogenicity by constructing recombinant chimeric viruses between HIV-1
and a non-pathogenic SIVagm originally isolated from African green
monkeys. METHODS: Starting from two infectious DNA clones, pNL432
(HIV-1) and pSIVagm3 (SIVagm3), two recombinant chimeric viruses have
been constructed. One of the chimeras, designated as HE-A391, has
HIV-1-derived env region (including tat, rev, vpu) with the rest of its
genome from SIVagm3; another, designated as SE-H13, has reciprocally
SIVagm3-derived tat, rev and env in the genome of HIV-1. RESULTS: Both
chimeric constructs yielded infectious viruses replicable in CD4
positive human cell line, M8166. Western blotting analysis revealed that
their structural proteins of both chimeric viruses were expressed as
designed. SE-H13 and HIV-1 were highly growth-competent with severe
cytopathic effect (CPE) in human PBMC whereas HE-A391 and SIVagm were
weakly replicable with no CPE. Similar results were obtained in other
human cell lines. On the other hand, the growth potency and
cytopathogenicity of the viruses were different in monkey PBMC where
SIVagm3 and HE-A391 were replicable with no CPE and HIV-1 and SE-H13
were weakly or only transiently replicable. In the case of SE-H13, not
CPE but severe cell deaths were observed. DISCUSSION AND CONCLUSIONS:
These results seem to suggest that the 5' half region of the virus
genome (LTR, gag and pol) is mainly important for the cytopathogenicity
as well as the cell tropism. These HIV-1/SIVagm3 chimeras will be useful
a variety of AIDS pathogenesis and vaccine studies.
DE Animal Cell Death Cell Line Cercopithecus aethiops Chimera
Comparative Study Genes, env Genes, rev Genes, tat Genome, Viral
Human HIV Long Terminal Repeat
HIV-1/GENETICS/*PHYSIOLOGY/*PATHOGENICITY Lymphocytes/MICROBIOLOGY
*Recombination, Genetic Repetitive Sequences, Nucleic Acid
SIV/GENETICS/*PHYSIOLOGY/*PATHOGENICITY Virus Replication MEETING
ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).